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Title: 2944 - Stimulation of Reparative Dentinogenesis Under Various Biomaterials in the Experiment

Authors:

Zarina Abdurakhmanova (Presenter)
Central Research Institute of Dental and Maxillofacial Surgery, Moscow, Russia

Alexey Volkov, Federal State Budgetary Institution "National Medical Research Center of Traumatology and Orthopedics named after N.N.Priorov", Moscow, Russia
Elena Krechina, Central Research Institute of Dental and Maxillofacial Surgery, Moscow, Russia

Abstract:

Objectives: To analyze the features of reparative dentinogenesis with the use of biomaterials in the accidental pulp exposure in the experiment.

Methods: In accordance with the objective of the study, an empirical experiment was conducted on 4 minipigs. The experiment was based on the Scientific Centre of Biomedical Technologies RAMS.
Each animal in the experimental group was subject to a traumatic tooth’s pulp exposure in the maxilla area to the right and left sides (3 premolars on the each side). Two biomaterials were accurately loaded on the exposed pulp horn: Biodentine (Septodont, France) and Aureoseal (OGNA, Italy) and FUJI IX (GC, Japan) covered the cavity. Totally 24 premolars. After first 2 weeks, 4 weeks, 12 weeks, and 6 months animals were euthanized and samples of jaw bones were taken.
Histological examination of the minipigs teeth was carried out using casting method in methyl methacrylate (OsteoBead, Sigma, USA). Slices of 35-50 micron were prepared from the samples and painted using the “Heaven Trichrome” method. Documentation was prepared using microscope Leica DM100 and digital camera EC3 in reflected and transmitted lights.

Results: Histological examination of the teeth’s slices revealed that in 2 weeks time after the pulp exposure and the application of Aureoseal and Biodentine the processes in the pulp camera were quite comparable. The proliferation of odontoblasts were detected on the side surface of the camera. Layers of new dentine and odontoblasts were distributed on the lower surface of the material with the formation of dentinal bridges by the 4 week of observations in both groups. By the 12 week there was a decrease in proliferative activity of odontoblasts and maturation of dentine in every cases; the volume of the pulp cavity decreased by third as a result of proliferation of odontoblasts. By the 6 month in the experiment no significant changes in the structure of the dentine and the pulp were detected.

Conclusions: The conducted morphological experiment has determined that the used biomaterials, facilitate the regeneration of dentine equally.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None

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