Title: 0810 - Influence of Different Dentifrices on Biofilm and Nails Fluoride Levels


Cristiane Cardoso (Presenter)
Cruzeiro do Sul University

Fernanda Campos, Cruzeiro do Sul University
Silmara Silva, Centro Universitário de João Pessoa
Andre Vieira, Centro Universitário de João Pessoa
José Viana Filho, Centro Universitário de João Pessoa
Marília Buzalaf, Bauru Dental School
Fábio Sampaio, Paraíba Federal University


Objectives: The aim of this this randomized clinical trial was to evaluate the effect of dentifrice pH and fluoride concentration ([F]) on fluoride uptake in biofilm and nails of children living in a non-fluoridated area.

Methods: A total of 228 two-to-four years old children were randomly allocated into 3 groups differing according to the type of dentifrice used over 12 months: G1: 1100ppmF, pH4.5 (n=76); G2: 750ppmF, pH4.5 (n=74); G3: 1100ppmF, pH7.0 (n=78). Nails were collected after 4, 8 and 12 months after starting the use of the dentifrices (T1, T2 and T3) and biofilm was collected 5 and 60 minutes after toothbrushing. The concentrations of F in nails and biofilm were analyzed by specific electrode after facilitated diffusion by HMDS. Considering nails, data were analyzed by ANOVA/Tukey’s test and regarding biofilm, a comparison between collection times was done by Wilcoxon test and among dentifrices by Kruskal Wallis/Mann-Whitney’s test (p〈0.05).

Results: The results showed a significant reduction of biofilm [F] 60 minutes after toothbrushing, regardless the dentifrice used. However, 5 minutes after toothbrushing, G1 had a significantly higher biofilm [F] compared to G2 and G3 and 60 minutes after toothbrushing, [F] was significantly higher for G1 and G2 compared to G3. Regarding nails, the results showed a significant difference between the groups in T1 and T2, but not in T3. However, considering the [F] of the three moments, G1 and G3 had significantly higher [F] in nails compared to G2.

Conclusions: It can be concluded that the pH reduction of the dentifrice leads to a higher incorporation of F in biofilm, which remains significantly higher 60 minutes after toothbrushing and the reduction of the dentifrice concentration is still a relevant factor for the reduction of excessive intake of fluoride.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None.