Title: 2830 - Analysis of Gingival Transcriptomes in Type 2 Diabetes and Aging
Oelisoa Andriankaja (Presenter)
University of Puerto Rico
Dana Graves, University of Pennsylvania
Kelvin Carrasquillo, University of Puerto Rico
Abiel Roche, University of Puerto Rico
John Novak, University of Kentucky
Sreenatha Kirakodu, University of Kentucky
Arnold Stromberg, University of Kentucky
Luis Orraca, University of Puerto Rico
Janis Gonzalez-Martinez, University of Puerto Rico
Jeffrey Ebersole, University of Kentucky
Octavio Gonzalez, University of Kentucky
Objectives: Older adults over the age of 65 years represent one of the fastest growing segments of the diabetes and periodontal disease population. Impaired inflammation is a common denominator in diabetes, aging and periodontal disease. Herein, we sought to identify gingival genes/pathways that are similarly affected in type 2 diabetes and aging-related periodontal disease.
Methods: Expression analysis of a selected group of 550 genes previously associated with periodontitis in type 2 diabetic Goto-Kakizaki rats (n=4-5), was evaluated in gingival tissues from young (<3 years) and aged (>15 years) rhesus monkeys (Macaca mulatta) (n=9/age group) using the ligature-induced periodontal disease model and microarray. Gene expression was evaluated at baseline (Health), 3 months after ligature placement (Periodontitis), and 2 months after removing the ligatures (Disease resolution). Comparative expression analysis was also developed for genes that were found to be regulated by a TNFα inhibitor in type 2 diabetic rats. A fold change of 1.75 was taken as cutoff point. Enrichment score ≥ 1.5 and p-values <0.05 were used to define criteria for functional annotation of gene clustering analysis.
Results: 1) There were a total of 266 up-regulated and 195 down-regulated genes in aged rhesus monkeys compared to the young group during the resolution, among which a total of 23 up-regulated and 73 down-regulated showed a similar trend in gene expression in diabetic rats treated by the TNF-inhibitor.
2) A functional annotation analysis from the overlapped genes between the rhesus monkeys and diabetic rat models mainly revealed up-regulation of host defense response and cell-signaling pathways and down-regulation of neuronal gene clusters.
Conclusions: Biologic pathways of neuronal and host defense responses are associated with gingival gene transcriptomes in both diabetes and aging during the resolution of periodontal disease.
This abstract is based on research that was funded entirely or partially by an outside source:
NIH: DE017732; P40RR03640; P20GM103538, and UL1TR000117
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: "NONE"