Title: 2177 - Anti-inflammatory Effects of GW0742, a PPARδ Agonist, on Pulp Cells
Caroline de Lima (Presenter)
University of Brasilia
Bruna Amorim, Universty of Brasilia
Carine Royer, University of Brasilia
Maria de Fátima Borin, University of Brasilia
Francisco Neves, University of Brasilia
Ana Acevedo, Universty of Brasilia
Objectives: Identification and characterization of molecules able to dampen inflammatory events within the dental pulp, facilitating dentinal repair, offer a potential to increase the success in maintaining the tooth vitality. Due to its anti-inflammatory properties, the peroxisome proliferator-activated receptors delta (PPARδ) could be envisioned as a putative therapeutic target. Thus, the aim of this study was to assess the anti-inflammatory ability of GW0742, a PPARδ full agonist, on human dental pulp cells (hDPCs).
Methods: For this, hDPCs were treated with lipopolysaccharide (LPS)/Hydrogen peroxide (H2O2) or LSP/H2O2 plus GW0742 (10 nM, 100nM ou 1 μM), and real time SYBRTM Green PCR was used to analyze cytokines (IL6; IL1β; MCP1) and metalloproteinases (MMP1,-2) mRNA expression. Also, a LPS or LPS plus GW0742 (1 μM) – conditioned hDPCs cultures were tested for their chemotactic effect on an immortalized murine macrophage cell line (RAW 264.7) in a transwell co-culture system.
Results: GW0742 repressed IL6, IL1β, MCP1 and MMP1 gene expression in LPS/H2O2 treated hDPCs (p < 0.05, with Kruskal-Wallis, followed by Dunn's Multiple Comparison Test). In addition, treatment with GW0742 significantly reduced the ability of LPS-stimulated pulp cells to recruit RAW 264.7 macrophages (p < 0.05, with one-way ANOVA, followed by Newman-Keuls Multiple Comparison Test).
Conclusions: GW0742 can modulate pulp inflammatory response, suggesting an anti-inflammatory effect of activated PPARδ receptor.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE