Title: 1625 - Biomarker for Apical Periodontitis in Gingival Crevicular Fluid: Pilot Study


Teodora Karteva, Medical University - Plovdiv
Neshka Manchorova-Veleva (Presenter)
Medical University - Plovdiv

Ekaterina Karteva, Medical University - Plovdiv
Donka Keskinova, University of Plovdiv Paisii Hilendarski
Stoyan Vladimirov, Medical University - Plovdiv


Objectives: Matrix metalloproteinase (MMP)-8 is a potential biomarker for periodontal tissue metabolism in gingival crevicular fluid (GCF). The aim of this pilot study was to determine the levels and diagnostic accuracy of active MMP-8 (aMMP-8) in GCF from teeth with asymptomatic apical periodontitis (AAP) and their contralateral teeth with a clinically healthy apical and marginal periodontium.

Methods: In this cross-sectional study, GCF samples were obtained from teeth with AAP (n=3) and their contralateral teeth as controls (n=3). The diagnosis of AAP and the determination of clinically healthy periodontal tissues were established with clinical and cone beam computed tomography parameters. The volume and characteristics of the lesions were assessed. Pool GCF samples per tooth were obtained from the whole crevice’s perimeter (mesiovestibular, vestibular, distovestibular, distolingual, lingual and mesiolingual). aMMP-8 levels were determined by ELISA. Results were evaluated based on direct comparisons of measurement data as due to the small sample size no statistical testing was performed.

Results: A trend towards higher concentrations of aMMP-8 in teeth with AAP was observed. The healthy controls demonstrated biomarker levels corresponding to a healthy periodontal tissue. Greater lesion volume also corresponded positively with higher aMMP-8 levels.

Conclusions: The results of this pilot study suggest that the GCF composition is modified by AAP and that the levels of aMMP-8 as a potential biomarker are related to the lesion’s volume.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE