Title: 3412 - Effect of Folic Acid in Mesenchymal Cell Differentiation In Vitro
Zita Bendahan Alvarez (Presenter)
Universidad El Bosque
Lina Escobar, Universityersidad El Bosque
Jaime Castellanos Parra, Universidad El Bosque
Ingrid Mora, Universidad El Bosque
María González, Universidad El Bosque
Objectives: Folic acid (FA) is the most stable synthetic form of folate (vitamin B) which when is administered during pregnancy prevents the apparition of birth defects. The effects of FA to prevent the formation of orofacial clefts remains controversial and its role in the differentiation of mesenchymal stem cells (MSCs) to osteoblasts is unknown and deserves further research.
Objective: To determine the effect of FA on differentiation of dental pulp MSCs to osteoblast.
Methods: MSCs obtained from dental pulp (DPSCs) were treated with FA (120-240 μM) for different time periods (24-144h) to assess changes in proliferation and viability by staining with resazurin and changes in morphology by phase contrast microscopy. To determine the FA effect on DPSCs differentiation process, the cells were seeded and treated with FA 160μM and 200μM or osteogenic induction medium (OIM) for 21 days. Finally, expression of differentiation genes was evaluated in each group by RT-qPCR using the Schefe method analysis. Quantitative variables were compared using ANOVA and multiple comparisons. A value of p<0.05 was considered significant.
Results: FA induced morphological changes in DPSCs with lower cell density and more elongated and spindle-shaped cells as the dose and treatment time increased. FA treatment induced a 10% of reduction in cell proliferation, although it was no significant regarding untreated control cells. Despite FA induced a downregulation of osteoblastic differentiation genes in the first treatment week, at the second treatment week the master transcription factor RUNX2 is upregulated leading to a significant increase in transcripts to ALP, OSX and OC, an expression change that disappear after three weeks of treatment.
Conclusions: FA promotes morphological changes and an increase in osteogenic genes expression after two weeks of treatment, suggesting that FA could be involved in DPSCs differentiation into mineralizing phenotype in vitro.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE