Title: 0962 - Bacterial Penetration of the Dental Implant Cementless Fixation System


Kung Kwon (Presenter)
Kyung Hee University

Seoung-Jin Hong, Kyung Hee University Dental Hospital
Eun-Young Jang, School of Dentistry, Kyung Hee University
Ji-Hoi Moon, School of Dentistry, Kyung Hee University


Objectives: Conventional dental implant prostheses are classified into three categories according to the retentive methods; screw-retained, cement-retained and screw and cement-retained prosthesis. Each type of the prosthesis has its own advantages and disadvantages. For each clinical situation, the appropriate prosthesis type is selected. Cementless fixation (CL.F) implant prosthesis is novel method had no biological complications induced by cement remnants and the integrity of the occlusal surface was maintained. The purpose of this study was to compare the number of bacteria that penetrate the abutment-implant interface, depending on the type of implant prosthesis.

Methods: Three types of prosthesis were used in this study; screw-retained prosthesis, cement-retained prosthesis, and CL.F prosthesis. Seven samples per group were disinfected with ethylene oxide gas and placed in a 50 ml tube. Prevotella intermedia ATCC49046 was grown in brucella broth. The bacterial culture adjusted to an optical density at 600 nm (OD600) of approximately 0.5 was added to the tube containing the samples, then further incubated for 48 hours with rotating. Prosthesis removed from the bacterial culture was washed with PBS before separating the crown and abutment except screw-retained prosthesis. The bacterial cells attached to the inside the crown and the abutment were washed with 500 μl of 0.85% NaCl and suspended. The bacterial cell counts were compared by measuring the bacterial viability and ATP-bioluminescence of the suspension using LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen) and BacTiter-Glo Microbial Cell Viability Assay kit (Promega).

Results: In both methods, number of the bacteria that penetrate into the abutment-implant interface was highest in screw-retained prosthesis, second highest in cement-retained prosthesis , and lowest in CL.F prosthesis.

Conclusions: CL.F implant prosthesis is less susceptible to bacterial adhesion and penetration than other types, which may be due to the absence of screw holes and precise fit.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE