Title: 0960 - Effects of Erbium-doped Yttrium Aluminum Garnet Laser on Lipopolysaccharides of Porphyromonas gingivalis Over Titanium Discs: An in vitro Study
Kuan Lun Fu (Presenter)
Dental School of National Taiwan University
Ya Ting Jhang, Dental School of National Taiwan University
Yi-Wen Chen, Dental School of National Taiwan University
Mark Yen-Ping Kuo, Dental School of National Taiwan University
Chen-Ying Wang, Dental School of National Taiwan University
Objectives: Studies have reported a high prevalence of peri-implantitis with the radiographic marginal bone loss. Evidence to date indicates that no available treatments result in total resolution of established peri-implantitis. The aim of this study was to mimic the biofilm contamination on titanium discs, and evaluate the anti-microbial effect of erbium-doped yttrium aluminium garnet (Er: YAG) laser irradiation on Porphyromonas gingivalis (P. gingivalis) and lipopolysaccharides (LPS).
Methods: Grade IV titanium discs were incubated with a suspension of P. gingivalis (ATCC® 33277TM) in brain heart infusion (BHI) broth. Six groups (n=6) with a total of 36 titanium discs were prepared. Group 1 was the negative control. Group 2 to 6, titanium discs were incubated with P. gingivalis biofilm. Group 3, curette was used to debride the biofilm. Group 4, 0.12 % Chlorhexidine was used for irrigation. Group 5, Er: YAG laser irradiation was performed at pulse energy 60 mJ and frequency 20 pulses per second to debride the biofilm. Group 6, Er: YAG laser irradiation was followed by curette debridement. After various treatments, the topography of the titanium discs was observed by scanning electron microscope, and the quantitation of LPS was performed by Limulus amoebocyte lysate (LAL) test.
Results: Laser treatment of the titanium discs did not result in visible microscope alterations. Chlorhexidine irrigation couldn’t remove bacteria or LPS efficiently. The residual bacteria and LPS was significantly lowest in the group with Er: YAG laser irradiation and curette debridement.
Conclusions: Mechanical debridement is crucial to remove the biofilm. Er: YAG laser would be a promising adjuvant therapy to treat peri-implantitis by removal of LPS without altering titanium surface. Further studies are required to evaluate the potential of osteoblast or fibroblast adhesion after Er: YAG laser treatment.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: none