Title: 0959 - Comparative Study of Bacterial Growth on Grooved and Smooth Healing Abutments


Ofer Moses, Tel Aviv University
Hagay Slutzky (Presenter)
Tel Aviv University

Carlos Nemcovssky, Tel Aviv University
Israel Lewinstein, Tel Aviv University
Miron Weinreb, Tel Aviv University
Hasan Zoabi, Tel Aviv University
Shlomo Matalon, Tel Aviv University


Objectives: The aim of this in-vitro study was to evaluate the growth of 2 bacterial strains (Fusobacterium nucleatum and Porphyromonas gingivalis) onto healing abutments, with 2 different surface macro-morphology: one completely smooth and the other groove-marked.

Methods: Twenty 5mm-high implant-healing abutments were equally divided into two groups: I: smooth surface, II: groove-marked. F. nucleatum and P. gingivalis bacteria were cultured on five sterilized healing abutments of each type for 48 hours at 37°C under anaerobic conditions, yielding four experimental groups. Subsequently, abutments were examined under scanning electron microscopy. Attached bacteria were counted on the four vertical quarters of the grooved abutments and the most 2 coronal millimeters of smooth abutments and counts were statistically analyzed by two-way ANOVA after application of square root transformation for normal distribution.

Results: Bacterial counts in the marking (grooved) areas of the abutments were 20 times greater for P. gingivalis and 100 times greater for F. nucleatum compared with the smooth surfaces of the abutments (P ≤ 0.0001).

Conclusions: Adherence of both bacterial strains was significantly enhanced within the grooves of the marked healing abutments, while very few bacteria adhered to the smooth zones between grooves and the smooth surface of the non-grooved abutments.
Gingival wound healing following second stage implant exposure or one stage implant insertion with connection of a smooth healing cap may be better with lower bacterial count. Further studies should elucidate the clinical relevance of these findings.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None