Title: 0958 - Oral Mucosa Response to Zirconia Dental Implants. A Pilot Study


Elisabeth Jacobi-Gresser, Implantology and environmental oral medicine. Private practice
Mariela Domingo, Universidad de Buenos Aires. Facultad de Odontología
Deborah Tasat, National University of General San Martin
María Paparella, Universidad de Buenos Aires. Facultad de Odontología
Daniel Olmedo (Presenter)
Universidad de Buenos Aires. Facultad de Odontología


Objectives: Metal-free zirconia dental implants are a therapeutic alternative to titanium implants. Nevertheless, further basic and clinical studies are necessary to evaluate long-term outcomes and technical and biological complications associated with their use. The aim of this descriptive pilot study was to evaluate tissue response of human oral mucosa adjacent to zirconia dental implants.

Methods: Forty-three biopsies of the supra-implant oral mucosa adjacent to two-piece zirconia dental implants (Zeramex® T, Dentalpoint AG, Zürich, Switzerland) were analyzed between 3 and 4 months post-implant placement. Nine implants were placed without a healing cap. The mucosa was clinically healthy in all cases. Biopsies were taken and fixed in 10% buffered formalin and embedded in paraffin. Histological sections were stained with H-E or Grenacher stain to facilitate the detection of potential particles. Histochemical techniques were performed to rule out the presence of melanin and iron pigmentation. Epithelial and connective tissue response, particularly the presence of inflammatory infiltrate and particulate material, were evaluated by light microscopy. In 14 cases, chemical composition of the particles was analyzed by energy dispersive x-ray spectroscopy (EDS).

Results: All cases, with and without healing cap, showed epithelial hyperplasia and dense chronic inflammatory infiltrate without multinucleated giant cells in the connective tissue. In addition, a fine particulate material was observed in the tissues. As shown by histochemical analysis, all samples tested negative for melanin, though some tested positive for iron. EDS analysis showed the presence of aluminum, iron, zirconium, chromium, silica, nickel, silver and gold.

Conclusions: All the analyzed cases exhibited the same histological features, regardless of the use of a healing cap. The presence of metals in the tissues, particularly aluminum, shows the need for further studies to evaluate the effect of these metals on peri-implant tissues, and their influence on the clinical course of metal-free zirconia dental implants.

This abstract is based on research that was funded entirely or partially by an outside source:
University of Buenos Aires - UBACyT20020150100032BA / 0020130100332BA and National Council for Scientific and Technological Research, Argentina - PIP 11220130100091

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE