Title: 0859 - Cytotoxicity of Commercial and Experimental Resin Modified Glass Ionomer Cements (RMGICs)


Amani Agha (Presenter)
Queen Mary University of London

Sandra Parker, Queen Mary University of London
EK Parkinson, Queen Mary University of London
Mangala Patel, Queen Mary University of London


Objectives: To evaluate the cytotoxicity of commercial and experimental resin-modified glass ionomer cements.

Methods: Two commercial materials Fuji Plus (GC, Japan) and RelyX Luting (3M-ESPE, USA), two control (home) materials formulated based on the commercial materials formulation and eight experimental compositions were included. The experimental liquids followed the same formulation as their corresponding commercials (F group following Fuji Plus and R group following RelyX Luting) but replacing HEMA with 100% HPM in R1 and F1, 70%/30% HPM/THFM in R2 and F2, 50%/50% THFM/HEMA in R3 and F3 and 70%/30% THFM/HEMA in R4 and F4. Two sets of experiments with different surface to liquid area were conducted. The first set (n=3) included all compositions (the two commercial materials with their corresponding home and experimental). The second set was performed only on Fuji Plus formulations and the samples had higher surface to liquid area (141.4 mm2/mL; compared to 47.13 mm2/mL in the first experiment). Samples were placed into wells containing culture Dulbecco's Modified Eagle Medium (DMEM) and incubated for 72 hours. Cytotoxicity of the materials extracts on normal human oral fibroblast line (NHOF-1) was tested using the MTT assay.

Results: All RelyX Luting materials (p<0.00001) and only F2 (p=0.028) showed less cell viability compared to the control medium in the first set of experiments. All RelyX Luting materials exhibited colour change of the DMEM medium (from pink to yellow), which indicates pH acidity. The second experiment was only performed on the Fuji Plus group. F3 and F4 neat aliquots showed similar effects on NHOF-1 cells when compared with the control medium (p ≥0.185).

Conclusions: RelyX Luting and its corresponding experimental materials exhibited acid diffusion, which clinically might cause damage to the pulpal tissue. Therefore, their composition needs attention and further modification. THFM containing Fuji Plus experimental compositions showed promising results in that they showed limited cytotoxicity and subsequently merit further investigations.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE