Title: 0875 - The 3D-printed Strontium-contained Calcium Silicate Scaffold for Bone Tissue Engineering
Tsui-Hsien Huang (Presenter)
School of Dentistry, Chung Shan Medical University; Dental Department, Chung Shan Medical University Hospital
Chia Tze Kao, School of Dentistry, Chung Shan Medical University; Dental Department, Chung Shan Medical University Hospital
M Liu, School of Dentistry, Chung Shan Medical University; Dental Department, Chung Shan Medical University Hospital
Ming You Shie, China Medical University
Objectives: There were several studies proved strontium (Sr2+) has pronounced effects on enhancing bone tissue regeneration and inhibiting bone resorption. In this study, we were to characterize the Sr elicited effects on human mesenchymal stem cells (hMSCs) in vitro using 3D-printed Sr-calcium silicate (Sr-CS) scaffold.
Methods: The Sr-CS raw materials were synthesized with solid-state reaction method and further fabricated highly uniform Sr-CS scaffolds with controlled structure via 3D bioprinter. The mechanical properties were evaluated. The biological behavior of hMSCs after seeding with different Sr-contained of CS porous scaffolds were systematically studied. The alkaline phosphatase (ALP) activity and osteogenic-related gene expression of hMSCs were evaluated by western blot assay.
Results: The compressive strength of Sr-CS scaffolds were well controlled in the range 10-25 MPa when their pore size varied from 300 to 500 µm. 3D-printed Sr-CS scaffolds could effectively enhance the adhesion and proliferation of hMSCs, and the proliferation rates of hMSCs on Sr-CS scaffolds were distinctively higher (p<0.05) than those in raw CS scaffolds prepared by the same method. In addition, the Sr and Si ions released from Sr-CS scaffold significantly promoted the alkaline phosphate (ALP) activity and osteogenic-related gene expression of hMSCs after cultured for 14 days. (p<0.05)
Conclusions: The present results indicated that 3D-printed Sr-CS scaffolds with a specific ionic combination and high mechanical strength that possessed the ability to effect osteogenic differentiation of hMSCs, indicating that they might be promising 3d scaffold for bone tissue regeneration.
This abstract is based on research that was funded entirely or partially by an outside source:
Minster of Science Technique (MOST)
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE