Title: 3276 - In Vitro Effect of Different Fluoride Products in Preventing Dental Caries Under Microbiological Assay


Adilis Alexandria, Universidade Federal do Rio de Janeiro
Thiago Vieira, Universidade Federal do Rio de Janeiro
Andrea Gonçalves, Universidade Federal do Rio de Janeiro
Lucio Cabral, Universidade Federal do Rio de Janeiro
Ana Maria Gondim Valença, Universidade Federal da Paraíba
Lucianne Maia (Presenter)
Universidade Federal do Rio de Janeiro


Objectives: The aim of this study was to investigate the caries preventive effect of different fluoride dentifrices (FD) and solutions (FS).

Methods: Bovine enamel samples (n=60) were exposed to the following treatments (n=10): CPP-ACP toothpaste (GC® America); CPP-ACP/NaF toothpaste (GC® America); 1% TiF4 solution; 1.36% NaF solution; Blank Control (BC, culture media without biofilm) and Grow control (GC, culture medium with biofilm). The half part of the enamel samples surfaces was protected and served as self-control. FD treatment consisted of a 5-min soak in 1.5ml/block of toothpaste/deionized water slurries (1:3 w/w). FS were applied over the enamel surface for 1 min. After the treatments, the enamel blocks were water rinsed. Then, the microbial assay challenge with a mixed bacterial biofilm growing (S. mutans and S. salivarius – FD and S. mutans, S. salivarius, Lactobacillus casei and Candida albicans - FS) was performed under a microaerophilic environment (24 h, 37°C). The percentage of surface microhardness loss (% SML) was evaluated. Differences among treatments were analyzed using one-way ANOVA and Tukey tests for FD and Kruskal-Wallis/Mann-Whitney for FS. Enamel alterations were analyzed through the evaluation of the percentage of surface microhardness loss (% SML) and scanning electron microscopy (SEM) images.

Results: None of FD treatments differed from GC (p>0.05). All FS tests were better than GC (p<0.05). NaF solution presented lower %SML compared to TiF4 solution (p<0.05). In SEM images, the exposed surface of FD and FS groups appeared homogeneous with little signs of surface demineralization. The higher surface alterations were observed in specimens of GC. A titanium dioxide film was observed on enamel surfaces that were treated with TiF4 solution.

Conclusions: The tested fluoride dentifrices did not present preventive effect against a higher microbiological cariogenic challenge. However, all fluoride solutions were able to inhibit the mineral loss, especially the NaF solution.

This abstract is based on research that was funded entirely or partially by an outside source:
CNPQ, grant number: 303535/2016-4

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None.