Title: 3272 - Caries Preventative Therapies and Regimens: An In-Situ Study
Mannaa Aldowsari (Presenter)
King Saud University
Marina Malinowski, University of Leeds
Simon M Strafford, University of Leeds
Monty Duggal, National University of Singapore
Jinous Tahmassebi, University of Leeds
Objectives: To compare the efficacy of MI Paste Plus (900-ppmF) combined with fluoride toothpaste 1450-ppm F as a regimen with Clinpro Tooth crème (950-ppm F) and fluoride toothpaste 1450-ppm F on de/remineralisation of the enamel.
Methods: Design: In situ randomised, cross-over study. Methods: Sterilised bovine enamel slabs, intact and with subsurface caries-like lesions, were prepared and worn by healthy volunteers (n=14). The study had four arms, 21-day duration each. Four pastes were tested (i) Placebo (0-ppm), (ii) 1450 ppm F, (iii) MI Paste Plus combined fluoride toothpaste 1450-ppm F, (iv) Clinpro Tooth crème with 950 ppm F. The pastes were randomly assigned and each subject crossed over to each paste after a seven-day washout period. Slabs were subjected to a five times/day cariogenic challenge (12% sucrose) with the subjects using one of the treatments. Volunteers dipped their appliances twice/day for 2 minutes, in a slurry of the toothpastes. For group 3, additionally, the appliance was dipped for 3 min in a slurry MI paste once/ day immediately following the second exposure to the slurry of 1450 ppm F. Further de/remineralisation were assessed using Quantitative Light-Induced Fluorescence (QLF) and Microhardness before and after the treatment period.
Results: QLF results for all test groups showed a significant reduction (p < 0.05) in lesion volume (ΔQ) compared to the placebo. No statistically significant differences were found between the test groups. The Microhardness results for all test groups showed a significant increase (p < 0.05) of enamel hardness compared to the placebo. No statistically significant differences were found between the test groups.
Conclusions: The use of MI Paste Plus in combination with 1450 ppm F toothpaste did not show a greater reduction of enamel subsurface lesions or decreased demineralisation of sound enamel in comparison to 1450 ppm F toothpaste and Clinpro alone in our model.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE