Title: 3269 - Short Term Anti-root Caries Potential of Oral Hygiene Protocols


Camila Zamperini (Presenter)
University of Illinois at Chicago

Irene Adeleke, University of Illinois at Chicago
Ana Karina Bedran-Russo, University of Illinois at Chicago


Objectives: With a rapidly growing aging population, there is increasing concern with sustaining a healthy natural dentition, particularly in long-term care facilities. Simplified oral hygiene care could benefit elderlies who often present cognitive and motor impairments. The aim of this study was to compare the anti-root caries effect of different oral hygiene protocols using a salivary microcosm model.

Methods: Human root dentin surfaces (n=25) were divided among the following treatments: 1) MI Varnish (V; GC America Inc); 2) MI Varnish followed by MI Paste One (V+PO; GC America); 3) MI Varnish followed by MI Paste Plus (V+PP; GC America); 4) MI Varnish followed by MI Paste One and MI Paste Plus (V+PO+PP); and 5) Artificial Saliva (AS; negative control). First, sterile specimens were coated with varnish (V; V+PO; V+PP; V+PO+PP) and kept in artificial saliva (20 h at 37 °C). Then, the varnish was removed and the specimens were daily treated with PO (2 min; 2×/day) and/or PP (3 min; 1×/day), according to experimental group. After first day treatment, specimens were incubated with unstimulated human saliva for 1 h at 37 °C. Afterwards, saliva was removed and the specimens kept with Mc Bain medium (37 °C and 5% CO2) for biofilm growth and caries formation. After 7 days, caries depth (CD) and porosity (P) were estimated by Rhodamine infiltration using confocal laser scanning microscopy. Data were statistically analyzed using ANOVA and post-hoc Games-Howell tests (α=0.05).

Results: Lesions depths ranged from 32 um to 266 um. There were statistically significant differences in the CD and P for all treatment groups when compared to AS (p < 0.05); and no differences among treatment groups (p > 0.05).

Conclusions: The different oral hygiene protocols present short term anti-root caries properties, with average decrease in lesion depth of 70%. Varnish alone was effective to reduce root caries formation.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None.