Title: 3268 - Bioavailability of Fluoride in Saliva After Application of BIOMIN F®


Jakov Modric (Presenter)
University of Witten/Herdecke

Maja Rybka, Witten/Herdecke University
Ouafaa Kouji, Witten/Herdecke University
Robert Hill, QMUL
Wolfgang Arnold, University of Witten/Herdecke
Ella Naumova, University of Witten/Herdecke


Objectives: During the past decade new fluoride formulations have been developed which may increase the fluoride bioavailability in the oral cavity. A recent formulation is bioactive glass in BioMin F®. It was the aim of this study to determine the bioavailability of fluoride in supernatant saliva and salivary sediment after application of BioMin F®.

Methods: Thirty two test subjects participated in this parallel study. They were divided into two groups of 16 subjects. Group one brushed with BioMin F® (F bioactive glass containing toothpaste; BIOMIN, London, UK), group two with a sodium fluoride containing toothpaste eurodont (MAXIM Markenprodukte, Pulheim, Germany). Saliva was collected before (T0), immediately after (T1), 30 (T2), 120 (T3) and 360 (T4) minutes after tooth brushing. All experiments were repeated three times. After a washout period of two weeks the groups repeated the brushing cycle. Salivary sediment and supernatant saliva were separated by centrifugation and the fluoride concentration was determined using a fluoride sensitive electrode (96-09 Orion, Thermo Electron, Beverly, MA, USA). The results were evaluated statistically with the non-parametric Mann-Whitney U test for independent variables and the Wilcoxon sign test for related variables.

Results: Fluoride concentration in supernatant saliva increased significantly immediately after tooth brushing and remained significantly higher until 30 minutes after tooth brushing. At T3 and T4 the fluoride concentration was back to the baseline value. In salivary sediment the fluoride concentration increased also significantly immediately after tooth brushing and was back to the baseline level after 30 minutes. Comparison of the fluoride concentration between both groups revealed a statistically significant higher fluoride concentration in sediment and supernatant saliva after NaF application.

Conclusions: From the results may be concluded that application of BioMin F® increases the fluoride bioavailability in the oral cavity for a short time. NaF results in higher fluoride concentrations.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None