Title: 3267 - Bioavailability of Fluoride on Different Oral Surfaces After Application of NAF and BIOMIN F®


Tom Kovalev (Presenter)
University of Witten/Herdecke

Katharina Schmuetsch, University of Witten/Herdecke
Robert Hill, QMUL
Wolfgang Arnold, University of Witten/Herdecke
Ella Naumova, University of Witten/Herdecke


Objectives: Bioavailability of fluoride in the oral cavity depends upon the fluoride formulation and their ability to adhere to oral surfaces. A recent new formulation is bioactive glass in BioMin F®. It was the aim of this study to determine the bioavailability of fluoride on different oral surfaces after application of NaF and BioMin F®.

Methods: Thirty two test subjects participated in this parallel study. They were divided into two groups of 16 subjects. Group one brushed with BioMin F® (F bioactive glass containing toothpaste; BIOMIN, London, UK), group two with a sodium fluoride containing toothpaste eurodont (MAXIM Markenprodukte, Pulheim, Germany). Saliva was collected before (T0), immediately after (T1), 30 (T2), 120 (T3) and 360 (T4) minutes after tooth brushing. All experiments were repeated three times. After a washout period of two weeks the groups repeated the brushing cycle. Saliva as collected from the surface of the tongue, palate, mouth floor and cheek and the fluoride concentration was determined using a fluoride sensitive electrode (96-09 Orion, Thermo Electron, Beverly, MA, USA). The results were evaluated statistically with the non-parametric Mann-Whitney U test for independent variables and the Wilcoxon sign test for related variables.

Results: Fluoride concentration increased significantly immediately after tooth brushing and remained significantly higher until 30 minutes after tooth brushing on the tongue, palate, and cheek surface but not on the mouth floor. At T3 the fluoride concentration was back to the baseline value. Comparison of the area under the curve of the fluoride concentration between both groups revealed a statistically significant higher fluoride concentration in all surfaces after NaF application.

Conclusions: From the results may be concluded that application of BioMin F® increases the fluoride bioavailability in the oral cavity for a short time. NaF results in higher fluoride concentrations.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None