Title: 3266 - The Combined Remineralization Potential of Arginine and Fluoride


Mohammed Nadeem Bijle (Presenter)
Faculty of Dentistry, The University of Hong Kong

Manikandan Ekambaram, Faculty of Dentistry, University of Otago
Cynthia Yiu, Faculty of Dentistry, The University of Hong Kong


Objectives: Incorporation of arginine might enhance the anti-caries effect of NaF. Thus, the objective of the study was to examine the remineralization potential of arginine (Arg) in NaF toothpaste.

Methods: Fifty enamel specimens allocated to five groups (n = 10) were subjected to artificial caries formation. A 10-day pH-cycling was performed to treat specimens as per group – [1]: 2% Arg – NaF, [2]: 4% Arg – NaF, [3]: 8% Arg – NaF, [4]: NaF and [5]: deionized water. The test solutions were subjected to pH measurement, fluoride estimation, Na-Cl element analysis using ICP-EOS and FTIR analysis. The specimen mineral density was assessed using Micro-CT, Ca/P ratio and surface fluorine concentration was determined using energy dispersive x-ray spectroscopy (EDS) and enamel fluoride uptake (EFU) by acid-etch method. Statistical analysis was performed using parametric/non-parametric tests with pairwise/multiple comparisons at p-value < 0.05.

Results: pH, fluoride concentration and Na-Cl ratio exhibited significant difference amongst groups (p < 0.001). FTIR analysis showed presence of free amino acids with 2% Arg-NaF. The mean mineral gain (0.40 ± 0.07g/cm3) and percent remineralization (27.91 ± 4.66%) for 2% Arg-NaF was significantly higher than other groups (p < 0.001). The median Ca/P ratio for 2% Arg-NaF (1.60) was significantly higher than DI water (1.53) (p = 0.029). The mean surface fluorine concentration for 2% Arg-NaF (1.51 ± 0.14%) was significantly higher than NaF (1.02 ± 0.28%) (p < 0.001). The EFU for 2% Arg-NaF (6.84 ± 1.59 µg/cm2) was significantly higher from NaF (5.22 ± 1.88µg/cm2) (p < 0.001).

Conclusions: Incorporation of 2% arginine into NaF toothpaste significantly increased the remineralization of artificial incipient enamel carious lesion when compared to NaF toothpaste. Incorporation of 2% arginine in NaF toothpaste might provide synergistic anti-caries effect given the proven prebiotic benefits of arginine in caries prevention and the demonstrated remineralization effect in the present in vitro study.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE