Title: 3265 - Reduced Enamel Demineralisation From a Calcium Silicate and Phosphates Toothpaste


Gareth Owens (Presenter)
Unilever Oral Care

Mark Long, Unilever Oral Care
Adam Limer, Unilever Oral Care
Andrew Joiner, Unilever Oral Care


Objectives: To evaluate the protective effects of calcium silicate and sodium phosphate salts plus fluoride containing toothpaste on dental enamel within an in vitro caries model.

Methods: Enamel blocks were prepared from bovine incisors and painted in acid-resistant varnish leaving an area 4x4mm of the enamel surface exposed. Prepared samples were irradiated using a neutron source to induce an abundance of 45Ca within the enamel tissue. Irradiated samples were divided into 3 groups (n = 12) and 45Ca specific activity calculated from a subset of the sample batch (n = 5). Each group was cycled through a series of simulated cariogenic challenges consisting of 1:2 (w/w) toothpaste:dH2O slurry (5min), acetic acid buffer at pH 5.0 containing phosphatase enzyme (1h), and neutral buffer at pH 7.0 (5min). Treatments consisted of Paste A (toothpaste containing calcium silicate and sodium phosphate salts plus 1450ppm F- as SMFP), Paste B (silica toothpaste containing 1450ppm F- as SMFP), and Paste C (fluoride-free silica control paste). Treatments were repeated for 12 cycles with 45Ca released into the acid buffer measured per mm2 of the exposed enamel.

Results: Results were expressed as mean release rates of tooth enamel in acid ( The mean (± S.D.) release rates for the enamel samples treated with Paste A, Paste B, and Paste C were 0.024 ± 0.056, 0.289 ± 0.069, and 0.366 ± 0.068 respectively. Statistical comparisons made using ANOVA (Tukey’s HSD post-hoc test) revealed significant differences between all treatment groups (p ≤ 0.018).

Conclusions: Enamel treated with the toothpaste containing calcium silicate, sodium phosphate salts and fluoride (Paste A) exhibited a significantly lower 45Ca release rate in acid and hence gave greater enamel protection under simulated cariogenic challenges than both fluoride and fluoride-free controls.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: Gareth Owens, Mark Long, Adam Limer, Andrew Joiner are employees of Unilever Plc.