Title: 3264 - Effects of Experimental Tooth Pastes Containing POs-Ca and/or Fluoride


Go Inoue (Presenter)
Tokyo Medical and Dental University

Amr Saad, Tokyo Medical and Dental University
Mahmoud Sayed, Tokyo Medical and Dental University
Tomoko Tanaka, Ezaki Glico Co., Ltd.
Hiroshi Takii, Ezaki Glico Co., Ltd.
Junji Tagami, Tokyo Medical and Dental University


Objectives: Phosphoryl-Oligosaccharides of Calcium (POs-Ca) is produced by enzymatic digestion of potato starch and has been compounded into a chewing gum (POs-Ca®, Ezaki Glico Co., Ltd., Osaka, Japan). And it has been reported that remineralization on subsurface lesion was detected with using this chewing gum. The aim of this study was to evaluate the effects of tooth pastes containing POs-Ca and/or fluoride on enamel.

Methods: Experimental tooth paste (4groups) were prepared by adding POs-Ca (13.2mM Ca) and/or fluoride (1450ppmF). A 3x3mm window on enamel of extracted human molar was prepared by nail varnish, and specimens were demineralized for 96hours to make subsurface lesion. Specimens were soaked in slurry with each tooth pastes diluted by 3times for 5min. Then pH cycling (30min Demin. and 150min Remin., 3times/day) was performed for 7days. Treated specimens were evaluated by Transverse microradiography (TMR) for mineral recovery and Confocal Laser Scanning Microscope (VK-X160) for surface roughness (Sa; arithmetical mean height of area). Sa values were statistically analyzed by two-way ANOVA (p=0.05).

Results: The combination with POs-Ca and fluoride showed the highest mineral recovery rate (66.9%) compared with control group (15.7%). However POs-Ca group did not show the higher recovery rate compared with fluoride group. There was no statistical differences in ΔSa (difference before and after pH cycling) among POs-Ca/fluoride group, POs-Ca, fluoride and control (-5.8nm, 17.9nm, 155.9nm and 82.5nm respectively)(n=6).

Conclusions: Addition of POs-Ca and fluoride in tooth paste could recover the mineral density significantly in enamel subsurface lesion. However, the effect of POs-Ca only group did not show enough recovery rate compared with fluoride only group. The changes after pH cycling was not detected in surface roughness analysis in all groups

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE