Title: 2382 - CALCIVIS Imaging System: Monitoring Erosive Lesion Treatment by Toothboost Technology


Graham Bailey (Presenter)

Bruce Vernon, Calcivis
Richard Willson, Modus Laboratories Ltd.
Carol Rafferty, Dentherapy Ltd


Objectives: To evaluate the capability of CALCIVIS® imaging system to monitor the re-mineralisation of erosive lesions and compare to microindentation. Measure the effectiveness of Toothboost® Technology as a remineralisation treatment.

Methods: The CALCIVIS imaging system consists of a sensitive intraoral camera capable of imaging low level, transient luminescence when a photoprotein is applied to enamel surfaces and reacts with free calcium ions resulting from enamel demineralisation. N=30 bovine enamel discs were imaged using a bench top version of the CALCIVIS imaging system before and after demineralisation (0.0475M citric acid pH3.75, 37oC, ten minutes).
Three groups of n=10 enamel specimens were treated five time a day for five days with one of: A. Toothboost delivered in an oral spray. B. (Positive control) 500ppm fluoride solution (MPF). C. (Negative control) deionised water.
Toothboost is a protein stabilised calcium rich formulation, delivered in various vehicles including an “on the go” frequent use oral spray.
Micro-indentation and luminescence measurements were made on the initial enamel, after demineralisation, and after treatment.

Results: The CALCIVIS imaging system clearly showed an increase in the luminescent signal after the erosive challenge. All enamel specimens treated with either Toothboost or fluoride solution then returned to a low background level. Treatment with deionised water showed reduction in luminescence but still clearly above the baseline level of the original enamel.
Luminescence: MFP 92.44% reduction SD8.0. Toothboost 84.66% reduction SD13.17. Water 35.22% reduction SD42.29.
Microindentation: MFP 25.39% re-hardening SD6.05. Toothboost 31.28% re-hardening SD6.37. Water 16.40% re-hardening SD8.08.

Conclusions: CALCIVIS imaging system shows visible result of demineralisation and monitors remineralisation using bioluminescence technology and shows good agreement with the established method of micro-indentation.
Toothboost provides an effective alternative remineralisation treatment for eroded enamel surfaces.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: G. Bailey and B. Vernon are employees of Calcivis who produce the imaging device used in the study. C. Rafferty is MD at Dentherapy who developed the Toothboost technology investigated in the study. Modus is an independent contract research company.