Title: 3260 - The Use of Calcein AM in Caries-infected Dentine Detection


Ahmed Almahdy (Presenter)
King Saud University

Malik Alabdulqader, King Saud University
Garrit Koller, Kings College London


Objectives: The aim of this study is to use Calcein as an aid for caries-infected dentine (CID) excavation.

Methods: Five freshly extracted carious molars were used in this study. Each tooth was sectioned into four slabs using a water-cooled circular diamond wheel. The slabs were assigned into four different groups: Negative control, Carisolv, Calcein and hand excavation (HE) group. In the negative control group, there was no excavation of carious tissue. In Carisolv, caries-infected dentine was excavated selectively with the aid of chemo-mechanical agent, Carisolv® gel (OraSolv AB, Sweden). Calcein AM Cell Viability Assay (R&D Systems, USA) was applied on the fresh carious-infected dentine. Then, the surface was exposed to light cure source using the EliparTM S10 LED curing light (3M ESPE USA). Tissue with fluorescence signal was excavated using spoon excavator. In HE group, all caries was excavated using spoon excavator.
The Vickers-hardness number (VHN) of 80 points located at the margins of the remaining dentine tissue was obtained using microhardness tester (INNOVATEST, Netherlands). The blue-green autofluorescence signal (GAF) from the same slaps was acquired using confocal laser scanning microscopy (Nikon C2+ system, USA). Using ImageJ software, the GAF signal from three areas at lesion margin was calculated in each slab. One-way ANOVA statistical analysis test was used to find the significant difference between the groups for both VHN and GAF signal with α value set at 0.05.

Results: The mean VHN for HE group (56.62±5.98) was significantly higher than other groups (p<0.05). No significant different was found in VHN of negative control (35.70±8.83), Carisolv (28.25±7.56) and Calcein (37.7±10.62) groups. In addition, negative control group showed significantly higher GAF when compared other groups (p<0.001). No significant different was found in the GAF between other groups.

Conclusions: The use of calcein in caries excavation resulted in selective CID removal.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None