Title: 3258 - Total Protein Concentration Versus Saliva Buffer Capacity


Barbara Tymczyna-Borowicz (Presenter)
Medical University of Lublin

Marta Nakonieczna-Rudnicka, Medical University of Lublin
Teresa Bachanek, Medical University of Lublin


Objectives: Proteins in the saliva influence saliva differential properties. Saliva contains, among others, glycoproteins, proline-rich proteins, histidine-rich proteins and enzymesAnalysis of the total protein concentration in the saliva in relation to the value and buffer capacity of the saliva.

Methods: The studies were conducted in the group of 116 people aged 20-54, who reported for treatment at the Chair and Department of Conservative Dentistry with Endodontics in Lublin. Protein concentration was performed with the use of Bradford method (Bradford reagent, cat. No B-6916Sigma). Saliva buffer capacity assessment was performed with the use of CRT buffer test (IvoclarVivadent, Liechtenstein). Statistical analysis was conducted with the use of Mann – Whitney test. Statistically essential were test values of p<0.05.

Results: The mean value of total protein concentration for the saliva with medium buffer capacity was 0.69 g/L (Median 0.70 g/L), with high buffer capacity 0.52 g/L(Median 0.40 g/L), with low buffer capacity 0.67 g/L (Median 0.58 g/L).
In the studied group the mean value of the total protein concentration was 0.59 g/L(Median 0.48 g/L). No essential differences were stated in the mean total concentration of protein in the saliva in relation to differential value of the saliva buffer capacity (Z = 1.80, p>0.05).

Conclusions: Total protein concentration in the saliva and saliva buffer capacity do not reveal any correlations. Further studies are recommended.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

Group Authors: Marta Nakonieczna-Rudnicka, Teresa Bachanek