Title: 0817 - Enamel Micro-crack Inspection Using Near IR Light Trans-illumination With Fluorescence-staining


Toshio Maseki (Presenter)
Nippon Dental University

Mei KOMOTO, The Nippon Dental University
Chie TOKITA, The Nippon Dental University
Seisuke Koshida, School of Life Dentistry
Masahiko Maeno, The Nippon Dental University
Yoichiro Nara, The Nippon Dental University


Objectives: Enamel micro-cracks can be often observed in many teeth of elderly. However, accurate clinical diagnosis for enamel micro-cracks is very difficult. The purpose of this study was to examine the availability of inspection for enamel micro-crack using near infrared light trans-illumination device (DIAGNOcam, KaVo) with fluorescence-staining.

Methods: 10 extracted human upper incisors with typical enamel micro-crack were selected and investigated by four different methods.
The labial surfaces of all tooth specimens were polished with a low-speed rotated polishing-brush. After that, the grayscale picture of each tooth specimen was taken by digital camera (D-5100, Nikon) under visible-light (VL), as control. Every tooth specimen was also inspected by DIAGNOcam (IR). In addition, each tooth specimen was stained by 0.25% indocyanine green solution for 10 sec and then examined by DIAGNOcam (ST), and also photographed by a digital camera under visible-light (ST-VL).
The digital images of all tooth specimens obtained by four different methods were compared with background enamel surface.
The difference in gray-value was measured using image analysis Image J software and data were analyzed using one-way ANOVA and Tukey’s q-test.

Results: The mean gray-values (S.D.) were VL: 44.6(7.6), IR: 91.3(11.2), ST: 111.5(11.1) and ST-VL: 45.9(8.1). The difference in gray-value was influenced significantly by the four methods at p<0.01. The value of ST was statistically larger than others at p<0.01 and IR indicated greater value than VL and ST-VL at p<0.01.

Conclusions: An inspection using near-infrared light trans-illumination with indocyanine green solution was effective to detect the presence of enamel micro-crack. The color of the enamel surface existing micro-crack was not influenced by means of indocyanine green solution application. The new inspection can be one of non-invasive chair-side diagnostic methods and it was effective to detect the presence of enamel micro-crack.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: none