Title: 0828 - Remineralizing Ability or Antibacterial Efficacy of Hydraulic Calcium-silicate Dental Cements When Used in Variable Capping and Apexification Cases
Salma Fathy (Presenter)
Tarek Elkhooly, National Research Centre
Abeer Abd El-Aziz, Mansoura University
Tayseer Maaly, Mansoura University
Objectives: To investigate both cell-dependent (with mesenchymal stem cells, MSCs) and cell-independent (with caries like dentine lesions) remineralizing ability of hydraulic calcium-silicate dental cements (Biodentine and Theracal), in addition to effect of Biotitania and Titania addition to Biodentine plus their antibacterial behaviour.
Methods: Cytotoxcicity and cell-dependent remineralizing ability following incubation of MSCs in direct contact with materials’ discs, alkaline phosphatase (ALP) levels were normalized to the total protein content of cells and measured spectro-photometrically. For cell-independent remineralizing ability, caries like lesions were introduced by pH cycling of prepared pulpal floor cavities of natural teeth. Ca and P were detected using SEM and EDS with reference to sound dentine in remineralizing solution. Effect of adding Biotitania and Titania with different percentages (0.5, 1.5 & 3%) within Biodentine on both cell-dependent and cell-independent remineralizing abilities. Antibacterial ability was estimated from growth curves of Strep. Mutans strains in direct contact with all materials.
Results: Biodentine showed the highest number of proliferating MSCs (278000.41 ± 4000.06, after 72 hours) and highest concentration of ALP (209.26 ± 7.17 μU/μg protein) of incubation. It also showed higher intestines of Ca and P ions. SEM of dentin beneath both materials showed filing of most intratubular areas with mineralized structure without significant difference. Biodentine showed the lowest slope (0.003 ± 0.0005) of Strep. Mutans strains growth curve after 18 hours although it was not statistically different to Theracal. Biodentine with 3% Biotitania showed statistically highest ALP activity with the lowest antibacterial efficacy.
Conclusions: Biodentine is preferred as a direct pulp capping material, it can more significantly induce cell differentiation and enrich the area with Ca ions for remineralization. In addition it had insignificantly higher antibacterial effect. Addition of Biotitania with 3% is most appropriate for cases where remineralisation is needed over the antibacterial ability.
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The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE