Title: 0402 - Lithium Contained S-PRG Cement Facilitated hDPSCs Migration and Functional Vascularity


Manahil Ali (Presenter)
Osaka University Graduate School of Dentistry

Motoki Okamoto, Osaka University Graduate School of Dentistry
Shungo Komichi, Osaka University Graduate School of Dentistry
Masakatsu Watanabe, Osaka University Graduate School of Dentistry
Yusuke Takahashi, Osaka University
Mikako Hayashi, Osaka University


Objectives: Cell migration is an important compartment of wound healing process in dentin-pulp complex. We reported that combination of Lithium ions and surface pre-reacted glass-ionomer (S-PRG/LiCl) cement induced reparative dentin formation through enhancing differentiation and mineralization of human dental pulp stem cells (hDPSCs) in vivo and in vitro. In this study, we evaluated the ability of Li+ to promote hDPSCs migration in vitro and assessed the presence of functional blood vessels that could aid the migration process in vivo.

Methods: This study was conducted under the approval of the Institutional Animal Care and Use Committee of Osaka University Graduate School of Dentistry (No. 28-013-0). Direct pulp capping was performed on the maxillary molars of 8-weeks old Wistar rats using S-PRG/LiCl cement or MTA (control). Then, histopathological evaluation was carried out using Masson Trichrome Staining (MTC). hDSPCs (Lonza, Switzerland) were cultured for migration using scratch wound healing technique in extracts of different concentration of LiCl (10,100,1000 mM) in S-PRG cement. Microscopic images were taken 24 hours after wound injury and analyzed using image J software. Statistical analysis was performed using ANOVA (Tukey Kramer post hoc test) (α=0.05).

Results: MTC sections of the experimental groups demonstrated the presence of diluted blood vessels in early stages after pulp capping and dark stained nuclei along the dentinal wall were observed similar to the control. While migration assay displayed significant promotion in 10 or 100 mM of S-PRG/LiCl specimens compared with the control containing media only.

Conclusions: S-PRG/LiCl cement promoted migration of the pulp cells and established functional blood vessels at pulpal injury site as a part of the healing process, but further investigations are needed to assess the mechanism. This novel combination has a promising future as a bioactive pulp capping agent.
This study was supported by JSPS KAKENHI #16K20453, #17K11704.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE