Title: 0157 - In vivo Periodontal Regeneration by Adipose-tissue Derived Multilineage Progenitor Cells
Venkata Venkataiah (Presenter)
Tohoku University Graduate School of Dentistry
Keisuke Handa, Tohoku University Graduate School of Dentistry
Masahide Takedachi, Osaka University
Shinya Murakami, Osaka University
Hanayuki OKURA, National Institute of Biomedical Innovation
Akifumi Matsuyama, National Institute of Biomedical Innovation
Masahiro Saito, Tohoku University Graduate School of Dentistry
Objectives: The ultimate goal of periodontal disease treatment is the reorganization of functional tissue by the application of stem cells, cytokines and extracellular matrices that can regenerate the lost periodontal tissue. Regeneration of periodontal tissues is being clinically possible by using autogenic transplantation of mesenchymal stem cells (MSC). However, autologous MSC transplantation is limited due to depending on age, systemic disease and tissue quality, thus precluding their clinical application. Therefore, we evaluated the efficacy of allogeneic transplantation of adipose-tissue derived multilineage progenitor cells (ADMPC) in a micro-mini pig periodontal defect model.
Methods: ADMPC were isolated from greater omentum of micro-mini pig and investigated for MSC marker expression. Immune-modulatory activity of ADMPC were assessed by treating with cytokine cocktail (IFNγ, TNFα and IL-6). Periodontal defect models were established in a micro-mini pig and transplanted with allogeneic or autologous ADMPC. Clinical assessment and computed tomography were used to evaluate the outcome of periodontal tissue regeneration.
Results: Flow cytometry analysis confirmed that ADMPC express moderate to high levels of MSC markers including CD44, CD73 and CD90. ADMPC exhibit osteogenic differentiation ability by calcified nodule formation, alkaline phosphatase staining and expression of osteogenic related genes in the induced cells. Adipogenic differentiation of ADMPC confirmed by the formation of lipid Droplets stained with Oil O red in the induced cells. ADMPC showed high expression of immune-suppressive factors GBP4 and IL1-RA upon treatment with cytokine cocktail. Allogeneic transplantation of ADMPC induced significant bone regeneration in micro-mini pig periodontal defect model and regeneration ability was comparable to those of autologous transplantation.
Conclusions: These results suggested that allogeneic ADMPC has an immune-modulation property and promoted periodontal tissue regeneration. Thus allogeneic ADMPC may be an alternative to autologous ADMPC for periodontal tissue regeneration therapy.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE