Title: 0627 - Assessment of Myeloperoxidase Activity at Different Force Levels In The Gingival Crevicular Fluid During Initial Phase of Orthodontic Tooth Movement


Honey Gurbaxani (Presenter)
Vspm dental college and research centre nagpur


Objectives: To assess the myeloperoxidase activity in gingival crevicular fluid by varying the effective force levels to 50, 75, 100 and 150 grams.

Methods: Three groups (I,II,III) with 10 patients each, requiring upper first premolar extraction were further divided into two subgroups namely A and B. Subgroups A of all three groups were used as control with a force application of 150 grams whereas subgroups B of the three groups used 50, 75 and 100 grams respectively. For the application and assessment of force, nickel titanium closed coil springs and Richmond Orthodontic Stress and Tension Gauge were used respectively. Two Ni-Ti closed coil springs, one on each side of maxillary quadrant, extending from molar tube hook to the power arm of canine bracket were used to retract the canines into the extraction spaces. Force levels were varied using the dontrix gauge. Micropipettes were used to collect GCF from the canine region while ELISA was used for the detection of MPO.

Results: It was found that at 2 hours, the mean optical density was maximum for group C i.e. 0.177 (0.018), followed by control group with value 0.048 (0.021), while it was similar for group A and B. After 7 days, the mean optical density was same for Control group and group B, while in group A it was 0.186 (0.019), followed by 0.244 (0.12) for group C. Further, at 14th day, the mean optical density shows maximum for control group and group A i.e. 0.674 (0.048) as compared to remaining two groups.

Conclusions: There is a highly significant increase in the myeloperoxidase levels in the gingival crevicular fluid at the 14th day after application of 150 grams of force.
The increase in myeloperoxidase levels can be correlated to the onset of inflammatory reaction in the periodontium preceding orthodontic tooth movement.
There is also a highly significant increase in the myeloperoxidase levels in the gingival crevicular fluid at 14th day after the application of 50, 75 and 100 grams.
This finding can lead to the conclusion that a minimum of 14 days are required for the beginning of orthodontic tooth movement to take place.


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Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE