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Title: 0130 - The Effect of αCGRP on Macrophages Polarization in Osseointegration

Authors:

Ying Yuan (Presenter)
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University

Qin Zhang, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University
Xinyuan Zhang, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University
Bin Wang, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University
Yingying Wu, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University
Ping Gong, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University
Lin Xiang, State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University

Abstract:

Objectives: M1/M2 polarization of macrophages has been reported to play important roles in regulating the bone formation and osseointegration. Several studies have shown that calcitonin gene-related peptide-alpha (αCGRP) may influence the macrophages polarization in inflammatory responses. Additionally, our previous studies proposed that αCGRP overexpression at peri-implant sites resulted in greater gains in osseointegration. Therefore, the purpose of this study was to elucidate the influence of αCGRP on macrophages polarization in osseointegration.

Methods: In vitro tests, bone marrow derived macrophages (BMDMs) from C57BL/6 or αCGRP-/- mice were obtained and then treated with LPS for M1-like activation, IL-4 for M2-like activation. Flow cytometry was used to identify the M1/M2 polarization. Real-time PCR were performed to evaluate inflammatory functions. To further investigate the role of αCGRP in vivo, mice were divided into wild-type group, αCGRP-/- group, or αCGRP-/- with αCGRP lentivirus transfection group. The inflammation and bone formation around implants in murine maxillae were observed and analyzed.

Results: In vitro, flow cytometry results demonstrated that αCGRP deficiency promoted the M1 polarization and inhibits the M2 polarization in BMDMs, which were consistent with pro-inflammation and anti-inflammatory cytokines expression levels in real-time PCR. In vivo, compared to αCGRP-/- group, knock-out group received αCGRP gene transfection displayed better osseointegration and lower inflammation levels.

Conclusions: Our results demonstrate that αCGRP may play roles in macrophages polarization. αCGRP deficiency might influence the osseointegration, and αCGRP recovery with lentivirus transfection might improve osseointegration and reduce inflammation infiltration in a continuous and effective manner.

Student Presenter

This abstract is based on research that was funded entirely or partially by an outside source:
National Clinical Key Specialty for Oral Implantology, National Natural Science Foundation of China (No. 81701007, 81571008), Postdoctoral Science Fountation of Sichuan University (No. 2017SCU12056) and Youth Science Foundation of West China Hospital of Stomatology of Sichuan University (No. 2016-11).

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE

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