Title: 0600 - Effect of Implant Surface Roughness and Material on Susceptibility of Human Gingival Cells to Inflammatory Stimuli


Oleh Andrukhov (Presenter)
Medical University of Vienna

Christian Behm, Medical University of Vienna
Alice Blufstein, Medical University of Vienna
Benjamin Pippenger, Institut Straumann
Andreas Moritz, Medical University of Vienna
Xiaohui Rausch-Fan, Medical University of Vienna


Objectives: Periimplantitis is an inflammatory disease leading to the destruction of soft and hard tissues around a dental implant. Similarly to periodontitis, the progression of periimplantitis is driven by an inflammatory immune response. The impact of implant surface material and roughness on inflammatory processes in periimplantitis are not entirely clear. Hence, we investigated how titanium and zirconia surfaces with different roughnesses influence the susceptibility of primary human gingival cells to different inflammatory stimuli.

Methods: Primary human gingival progenitor cells were isolated from 7 healthy individuals. Cells were cultured on following surfaces: smooth titanium machined surface (TiM), smooth zirconia machined surface (ZiM), moderately rough titanium sandblasted acid-etched surface (SLA), zirconia sand-blasted acid-etched surface (ZLA) and stimulated with either lipopolysaccharide (LPS) or interferon (IFN)-γ. Resulting production of interleukin(IL)-6, IL-8, and monocyte chemoattractant protein 1 (MCP-1) was measured by qPCR and ELISA.

Results: Cells grown on zirconia surfaces exhibited generally lower cytokine production compared to cells grown on Ti surfaces. Upon IFN-γ stimulation, significantly lower levels of IL-6 were found on ZiM and ZLA surfaces compared to TiM and SLA surfaces, respectively. Furthermore, IL-8 levels were significantly lower on ZLA surface compared to SLA surface. Upon LPS stimulation, significantly lower IL-6 levels were observed on ZiM and ZLA surfaces compared to TiM and SLA surface, respectively. The surface roughness had a limited impact on production of pro-inflammatory mediators by gingival cells. Cells grown on smooth surfaces tended to exhibit higher levels of proinflammatory mediators compared to moderately rough surfaces. Significantly higher levels of IL-8 were observed for TiM surface compared to SLA surface upon stimulation with IFN-γ.

Conclusions: Both, implant surface material and roughness influence susceptibility of primary gingival cells to inflammatory stimuli. Zirconia material could be an important factor associated with lower susceptibility to periimplantitis.

This abstract is based on research that was funded entirely or partially by an outside source:
ITI Foundation, Grant No 1199_2016

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None