Title: 0599 - A Murine Peri-implantitis Model: Characterization and Comparison to Periodontitis


Einat Varon-Shahar (Presenter)
faculty of dental medicine, Hebrew university

Ariel Shuesterman, hebrew university, faculty of dental medicine
Ervin Weiss, Tel-Aviv University
Yael Houri-Haddad, Hebrew University - Hadassah School of Medicine


Objectives: The aim of this study was to develop a murine model for bacteria-induced alveolar bone loss around titanium implants, and to study the differences in gene expression and alveolar bone loss around implant in various conditions compared to those around teeth

Methods: Screw-shaped titanium implants, smooth surface or SLA coated, were inserted immediately after extraction of the first upper left molar, in 5-6-week-old BALB/c mice. The mice were infected with Porphyromonas gingivalis and Fusobacterium nucleatum 21 (early infection) or 42 days (delayed infection) after implantation. Six weeks post infection, bone volume around implants and teeth was compared by using micro CT, histological analysis was performed and differences in gene expression was compared by performing RNA sequencing on extracted mRNA from the tissue surrounding the implants and the teeth

Results: The bacterial infection induced alveolar bone loss around the teeth and the implants. A difference was noticeable in the survival rate of immediately infected implant (60%) compared to delay infected (100%). The level of bone loss was significantly higher around SLA and smooth surface implants compared to teeth and compared to the control group. There is no significant difference between the early and the delayed infection in alveolar bone loss level around the implants. A difference was found in the gene expression between the control and infected group for implants and teeth

Conclusions: The results show that the alveolar bone resorption around implants is higher compared to teeth, which may indicate a more severe infection reaction around implants by the same pathogens. In addition the results might indicate that a delayed infection method is superior for a model of osseointegration and induced peri-implantitis. This research opens new possibilities of studying peri-implantitis, and understanding its pathogenesis and genetics

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: none