Title: 0118 - Characterisation of Alternative Bone Graft Materials for Cleft Palate Repair


Mehdi Alrubayee (Presenter)

Mohammad Islam, Dundee dental School
Peter Mossey, University of Dundee


Objectives: Cleft lip/palate (CL(P)) is a common congenital craniofacial deformity with an average worldwide prevalence of 1 per 1000 live births, and requires lifelong surgical and non-surgical interventions. Alveolar bone grafts (ABG) at the mixed dentition stage are used in cleft repair and autologous bone is still regarded as the gold standard. However, donor-site morbidity is the major drawback. Therefore, synthetic substitutes are emerging as possible alternative ABG materials.
The purpose of this in vitro study was to develop a novel in vitro osteogenic progenitor cell-based model and to investigate the role of various calcium phosphate-based (CaP) materials in osteogenic differentiation.

Methods: Human embryonic palatal mesenchymal cells (HEPM) and normal gingival fibroblasts (MM1) were seeded on four (A, B, C, D) CaP scaffolds (Kuros Biosciences BV, The Netherlands), in α-Minimal Essential Medium (α-MEM) supplemented with 10% foetal calf serum (FCS) with/without DAG (dexamethasone, ascorbic acid and β-glycerophosphate) and/or bone morphogenic protein-2(BMP2) osteogenic reagents, for 21 days and analysed for biocompatibility and osteodifferentiation at different time points. Cell adhesion was investigated microscopically after staining the cells with methylene blue. Viability/proliferation was assessed by MTT assay, while expression of the osteogenic differentiation marker RUNX2 was visualised by immunofluorescence assay and quantified by using Image-J software.

Results: HEPM and MM1 cells were well attached to the materials. Material B stimulated cell proliferation more than other materials. HEPM expressed RUNX2 when cultured with the materials at different levels in the presence/absence of DAG and BMP2. In general, RUNX2 was expressed within 3 days and peaked on day 7. Material-wise, material D stimulated a higher expression level of RUNX2 than the other materials. However, in the presence of DAG, RUNX2 expression was significantly enhanced.

Conclusions: These data suggest that CaP scaffolds were suitable for expansion and osteogenic differentiation of HEPM and were therefore promising candidates as ABG alternative.

Student Presenter

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE