Title: 0470 - Prime-&-Rinse Approach Increases Dentin MTBS of Self-Etch and Universal Adhesives


Baiping Fu (Presenter)
Zhejiang University

Wenting Wang, Zhejiang University


Objectives: The study investigated the effect of prime-&-rinse approach on dentin bond strengths when a novel primer containing 15% 10-methacryloyloxydecyl dihydrogenphosphate and 1% benzalkonium chloride (MDP-BAC).

Methods: One-step self-etch (Clearfil S3 Bond, (S3)) and universal (Scotchbond Universal (SU), All-Bond Universal (AU)) adhesives were used in this study. Forty-five freshly-extracted human third molars were used in this study. Mid-coronal dentin surfaces were exposed and wet-polished. Fifteen dentin surfaces were primed with MDP-BAC for 15 s, water-sprayed for 30 s and gently air-dried (prime-&-rinse approach). Another fifteen dentin surfaces were primed with MDP-BAC for 15 s, and strongly air-dried for 5 s (reverse two-step self-etch approach). Fifteen dentin surfaces served as control (one-step self-etch approach). The dentin surfaces were applied with adhesives S3, SU and AU following the manufacturers’ instructions. Composite resins from the same manufacturer were placed on the pre-treated dentin surfaces. Samples were prepared into multiple micro-beams for microtensile bond strength (MTBS) tests. Resin-dentin interfaces were analyzed with transmission electron microscopy (TEM). Data were analyzed by two-way ANOVA and Tukey's test (P<0.05).

Results: Prime-&-rinse approach using MDP-BAC produced the highest dentin MTBS for all adhesives (P<0.01). Compared with the control group (one-step self-etch approach), reverse two-step self-etch approach could significantly increase dentin MTBS for all adhesives (P<0.05). TEM findings revealed that the dentin smear layer was completely removed by prime-&-rinse approach.

Conclusions: Prior to the application of one-step self-etch and universal adhesives, prime-&-rinse approach using MDP-BAC could greatly increase the dentin MTBS. The prime-&-rinse approach using novel primer MDP-BAC could be used as a supplement to the contemporary self-etch approach.

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE