Title: 0578 - Development of Chitosan Spheres Loaded With Calcium Phosphate


Flavia Gonçalves (Presenter)
University Ibirapuera

Diana Lopes, University Ibirapuera
Maria Moreira, University of São Paulo
Roberto Braga, University of São Paulo
Luiz Henrique Catalani, University of São Paulo


Objectives: Several biomaterials are used in dentistry to reach the neoformation of bone and periodontal tissues. The development of a biomaterial which shows, not only osteoconduction and oteoinduction properties, but also antimicrobial activity, is very promising in dentistry. The aims of this study were: to develop a method to synthetize chitosan particles and chitosan loaded with dicalcium phosphate dihydrate; and to characterize these particles so that they can be used in the future in bone and periodontal regeneration.

Methods: Chitosan solutions of 10mg/mL were prepared, associated or not to 2.5mg/mL of dicalcium phosphate dihydrate, in a 90% acetic acid. The solutions were eletrosprayed in three different flow rates, 0.2, 0.5 and 1.0 mL/h. The particles generated were characterized for scanning electronic microscopy, light scattering and thermogravimetric assay.

Results: It was observed that the three conditions presented two shapes of particles: one with small size, solid and spherical shape; and the other, larger, with a biconcave spherical shape. The condition of 0.5ml/h and 1.0mL/h showed the largest range of size dispersion, from 250 to 1500nm and from 300 to 2000 nm whereas the 0.2mL/h showed the narrowest, from 300 to 550nm. The zeta potential for chitosan and chitosan/dicalcium phosphate dihydrate particles were respectively 0.7 and 4.2 mV in the 0.2mL/h flow rate condition, 54 and 27mV in 0.5mL/h flow rate condition, and 25,4 and 28mV in the 1.0mL/h condition. Furthermore, the intermediary flow rate of 0.5ml/h was more efficient in the calcium phosphate incorporation, having 24% of inorganic content on the particles, against 10% of the others groups.

Conclusions: According to the exposed, the electrospray of chitosan and chitosan/dicalcium phosphate dihydrate solution with a flow rate of 0.5 mL/h was more effective in the formulation of biomaterials composed of chitosan loaded with dicalcium phosphate dihydrate.

This abstract is based on research that was funded entirely or partially by an outside source:
FAPESP (Fundação de Ampara à Pesquisa do Estado de São Paulo) - 2016/13114-2

Disclosure Statement:
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: None