Title: 0347 - Physicochemical and Antimicrobial Properties of Triclosan and Chlorhexidine Glass-Ionomer Cements
Jack Panko, Cardiff University
Victoria Groves, Cardiff University
Alastair Sloan, Cardiff University
Paul Milward, Cardiff University
Wayne Nishio Ayre (Presenter)
Objectives: The objective of this study was to investigate how incorporating triclosan and chlorhexidine into glass-ionomer cements (GICs) influence physicochemical and antimicrobial properties.
Methods: Triclosan and chlorhexidine were incorporated at 0, 1, 2, 5 and 10%w/w into commercially available GICs (Aquacem and Vitremer). Changes in shade, contact angle, Vickers hardness and compressive, flexural and diametral tensile strength were recorded. Antimicrobial release was assessed using high-performance liquid chromatography at 6, 24, 48, 72 and 96 hours, mathematical modelling to elucidate release mechanisms and micro-computed tomography to assess water penetration. Antimicrobial activity against Streptococcus anginosus and Enterococcus faecalis was assessed using disc diffusion assays and broth cultures with release supernatants. Fourier transform infrared spectroscopy was used to analyse chemical composition and scanning electron microscopy was employed to observe fracture surfaces and surfaces of release samples. A two-tailed test was performed to establish statistically significant differences between groups.
Results: Increasing the concentration of triclosan and chlorhexidine enhanced the antimicrobial properties of the GICs tested, however resulted in significant (p<0.05) reductions in compressive strength, flexural strength and diametral tensile strength. These reductions were more pronounce when using triclosan in water-based Aquacem and when using chlorhexidine in resin-based Vitremer, indicating hydrophobic-hydrophilic incompatibilities influence mechanical stability. Vickers hardness was comparable to the control up until 10%w/w concentrations of triclosan/chlorhexidine. Triclosan increased the lightness of shade and hydrophobicity of the material as concentration increased. Antimicrobial activity was observed for concentrations of 2%w/w and greater for both GICs with triclosan and chlorhexidine. Antimicrobial release mirrored previously measured fluoride release with release mechanisms linked to water absorption and dissolution.
Conclusions: GICs modified with triclosan and chlorhexidine demonstrated favourable antimicrobial properties however at the expense of mechanical performance. Incompatibilities between antimicrobials and setting chemistry highlight the need for more sophisticated delivery systems.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE