Title: 1221 - Salivary Glycoproteins of 7-year-old Caries Free and Caries Active Children
Yan Wang (Presenter)
Shanghai Stomatological Hospital
Xiao li Zeng, Shanghai Stomatological Hospital
Ying Zhang, Shanghai Stomatological Hospital
Objectives: To detect the level of glycoproteins in unstimulated whole saliva of 7 years old caries active children via sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDS-PAGE）analysis, and compare with that of caries free children.
Methods: 2ml unstimulated whole saliva were collected from fifty caries free(dmft/DMFT＝0) and fifty caries active(dmft/DMFT≥6) children aged 6-7 years old. The supernatant of the saliva were used to do SDS-PAGE protein electrophoresis. Then GENMED kits were used to stain glycoproteins. The number of stained glycoprotein band of each subject was recorded respectively. SPSS 20.0 software package was used for statistical analysis. Mann-Whitney U test was used to determine if there is significant differences between caries free group and cares active group.
Results: The number of glycoprotein bands in caries active group was significantly higher than that of the caries free group（P<0.01）.
Conclusions: The level of glycoproteins in unstimulated whole saliva of 6-7 years old caries active children was significantly higher than that of the caries free children. It was suggested that enhanced level of glycoproteins in saliva may have a potential relationship with increased caries incidence in children aged 6-7 years old.
The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE